Identification of very-long-chain polyunsaturated fatty acids from Amphidinium carterae by atmospheric pressure chemical ionization liquid chromatography-mass spectroscopy

Phytochemistry. 2008 Sep;69(12):2391-9. doi: 10.1016/j.phytochem.2008.06.011. Epub 2008 Jul 25.


A method is described for the enrichment of very-long-chain polyunsaturated fatty acids (VLCPUFAs) from total fatty acids of Amphidinium carterae and their identification as picolinyl esters by means of microbore liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The combination of argentation TLC and LC-MS/APCI was used to identify unusual VLCPUFAs up to hexatriacontaoctaenoic acid. Two acids, 36:7n-6 and 36:8n-3, were also synthesized to unambiguously confirm their structure. The possibilities of VLCPUFAs biosynthesis are proposed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dinoflagellida / chemistry*
  • Environment
  • Fatty Acids, Unsaturated / analysis*
  • Fatty Acids, Unsaturated / chemistry
  • Mass Spectrometry
  • Models, Molecular
  • Seawater
  • Spectrophotometry, Infrared
  • Spectrophotometry, Ultraviolet


  • Fatty Acids, Unsaturated