Metabolomic Profiling of Drosophila Using Liquid Chromatography Fourier Transform Mass Spectrometry

FEBS Lett. 2008 Aug 20;582(19):2916-22. doi: 10.1016/j.febslet.2008.07.029. Epub 2008 Jul 25.

Abstract

Hydrophilic interaction chromatography (HILIC) interfaced with an Orbitrap Fourier transform mass spectrometer (FT-MS) was used to carry out metabolomic profiling of the classical Drosophila mutation, rosy (ry). This gene encodes a xanthine oxidase/dehydrogenase. In addition to validating the technology by detecting the same changes in xanthine, hypoxanthine, urate and allantoin that have been reported classically, completely unsuspected changes were detected in each of the tryptophan, arginine, pyrimidine and glycerophospholipid metabolism pathways. The rosy mutation thus ramifies far more widely than previously detected.

Publication types

  • Validation Study

MeSH terms

  • ATP-Binding Cassette Transporters / genetics*
  • Animals
  • Chromatography, Liquid / methods*
  • Drosophila Proteins / genetics*
  • Drosophila melanogaster / genetics*
  • Drosophila melanogaster / metabolism*
  • Female
  • Fourier Analysis
  • Male
  • Mass Spectrometry / methods*
  • Mutation

Substances

  • ATP-Binding Cassette Transporters
  • Drosophila Proteins
  • bw protein, Drosophila