HIV-1 expression induces tubular cell G2/M arrest and apoptosis

Ren Fail. 2008;30(6):655-64. doi: 10.1080/08860220802134672.


Human renal biopsy studies suggest the presence of HIV-1 and associated signs of injury in renal tubular epithelial cells. Because renal epithelial cells lack conventional HIV-1 receptors, the modus operandi of HIV-1 in the induction of tubular cell injury remains a mystery. In the present study, we evaluated the role of HIV-1 gene expression in human proximal tubular cell apoptosis and cell cycle progression. HIV-1- or vector-transduced cells were assayed for cellular injury and cell cycle defect. HIV-1-transduced cells showed the progressive loss of viability in a time-dependent manner. Similarly, HIV-1-transduced cells showed greater apoptosis when compared with vector-transduced cells. A higher number of HIV-1 expressing cells showed cell cycle arrest at G2/M phase and enhanced tubular cell expression of phospho-p53(ser15), phospho-cdc-2(Tyr 15), and phospho-chk-2 (Thr 68). These findings suggest that in addition to the activation of apoptotic pathway, HIV-1-induced G2/M arrest may also contribute to tubular cell injury.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural

MeSH terms

  • Apoptosis / genetics*
  • Cell Communication / genetics
  • Cell Cycle / genetics
  • Cell Death / genetics
  • Cell Division* / genetics
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Epithelial Cells / pathology
  • Epithelial Cells / virology
  • Flow Cytometry
  • G2 Phase* / genetics
  • Gene Expression Regulation, Viral
  • HIV-1 / genetics*
  • Humans
  • In Situ Nick-End Labeling
  • Kidney Tubules / pathology*
  • Kidney Tubules / virology*
  • Transduction, Genetic