Mechanisms and methods in glucose metabolism and cell death

Methods Enzymol. 2008;442:439-57. doi: 10.1016/S0076-6879(08)01422-5.


Glucose metabolism represents a critical physiological program that not only provides energy to support cell proliferation, but also directly modulates signaling pathways of cell death. With the growing recognition of regulation of cell death by glucose metabolism, many techniques that can be applied in the study have been developed. This chapter discusses several protocols that aid in the analysis of glucose metabolism and cell death and the principles in practicing them under different conditions.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Apoptosis / physiology*
  • Carbohydrate Metabolism / drug effects
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone / pharmacology
  • Cell Death / physiology
  • Dehydroepiandrosterone / pharmacology
  • Glucose / metabolism*
  • Glucose Transporter Type 1 / metabolism
  • Humans
  • Proto-Oncogene Proteins c-bcl-2 / metabolism


  • Glucose Transporter Type 1
  • Proto-Oncogene Proteins c-bcl-2
  • Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
  • Dehydroepiandrosterone
  • Glucose