Quick identification of effective small interfering RNAs that inhibit the replication of coxsackievirus A16

Antiviral Res. 2008 Dec;80(3):295-301. doi: 10.1016/j.antiviral.2008.06.017. Epub 2008 Jul 26.


Coxsackievirus A16 (CA16) is a major causative agent of hand, foot, and mouth disease (HFMD). It can cause myocarditis, pericarditis and fatal shock. There is no effective therapy against CA16. RNA interference (RNAi) is a powerful tool to silence gene expression. The small interfering RNA (siRNA) that induces RNA degradation has recently been used as an anti-virus agent to inhibit virus replication. In this study, we established the complete nucleotide sequence of CA16 strain Shzh05-1, and then compared the nucleotide sequences of Shzh05-1 with sequences of other CA16 strains in GenBank. We chose conserved regions between Shzh05-1 and the two other CA16 strains to design 30 siRNAs and construct siRNA-encoding plasmids. Thirteen siRNAs targeting conserved regions of the virus could effectively block replication of CA16 in cultured cells. Combination transfection of these 13 effective siRNAs could also produce a high inhibitory effect. These strategies and results suggest that RNAi has potential therapeutic use for suppression of CA16 infection.

MeSH terms

  • Animals
  • Cell Line
  • Chlorocebus aethiops
  • Dose-Response Relationship, Drug
  • Enterovirus B, Human / genetics*
  • Enterovirus B, Human / metabolism
  • Genetic Therapy
  • Genetic Vectors / genetics
  • Hand, Foot and Mouth Disease / genetics*
  • Hand, Foot and Mouth Disease / therapy*
  • Hand, Foot and Mouth Disease / virology
  • Humans
  • Leukocytes, Mononuclear / drug effects
  • RNA Interference
  • RNA, Small Interfering / genetics*
  • Vero Cells
  • Virus Replication*


  • RNA, Small Interfering