Abstract
Phage display of protein and peptide libraries offers a powerful technology for the selection and isolation of ligands and receptors. To date, the technique has been considered limited to soluble, non-membrane proteins. We report two examples of phage display of full-length, folded and functional membrane proteins. Consistent display required the recently reported KO7(+) helper phage. The two proteins, full-length caveolin-1 and HIV gp41, display well on the surface of the phage, and maintain their binding activities as shown by in vitro assays.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Bacteriophages / chemistry
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Caveolin 1 / chemistry
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Caveolin 1 / metabolism
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HIV Envelope Protein gp41 / chemistry
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HIV Envelope Protein gp41 / metabolism
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Humans
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Membrane Proteins / chemistry*
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Membrane Proteins / immunology
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Membrane Proteins / metabolism
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Models, Molecular*
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Peptide Library
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Structure-Activity Relationship
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Viral Matrix Proteins / chemistry*
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Viral Matrix Proteins / immunology
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Viral Matrix Proteins / metabolism
Substances
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Caveolin 1
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HIV Envelope Protein gp41
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Membrane Proteins
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Peptide Library
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Viral Matrix Proteins
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gp41 protein, Human immunodeficiency virus 1