Paradoxical sleep deprivation impairs spatial learning and affects membrane excitability and mitochondrial protein in the hippocampus

Brain Res. 2008 Sep 16;1230:224-32. doi: 10.1016/j.brainres.2008.07.033. Epub 2008 Jul 17.

Abstract

Previous research has demonstrated that paradoxical sleep has a key role in learning and memory, and sleep deprivation interferes with learning and memory. However, the mechanism of memory impairment induced by sleep deprivation is poorly understood. The present study investigated the effect of paradoxical sleep deprivation (PSD) on spatial learning and memory using the Morris Water Maze. Effects of PSD on CA1 pyramidal neurons in hippocampus were also examined. PSD impaired spatial learning of rats. PSD induced translocation of Bax to mitochondria and cytochrome c release into the cytoplasm, and decreased the membrane excitability of CA1 pyramidal neurons, effects which may contribute to the deficits in learning behavior. These results may partially explain the mechanism of the effect of PSD on learning. Modulating the excitability of hippocampal neurons and protecting mitochondrial function are possible targets for preventing the effects of paradoxical sleep deprivation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Body Weight / physiology
  • Cytochromes c / metabolism
  • Cytoplasm / enzymology
  • Cytoplasm / metabolism
  • Data Interpretation, Statistical
  • Electroencephalography
  • Hippocampus / metabolism*
  • Hippocampus / physiopathology*
  • Male
  • Maze Learning / physiology*
  • Memory / physiology
  • Mitochondrial Membranes / physiology*
  • Mitochondrial Proteins / metabolism*
  • Organ Size / physiology
  • Protein Transport / genetics
  • Protein Transport / physiology
  • Psychomotor Performance / physiology
  • Pyramidal Cells / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Sleep Deprivation / physiopathology*
  • Sleep Deprivation / psychology*
  • Sleep, REM / physiology*
  • bcl-2-Associated X Protein / metabolism

Substances

  • Mitochondrial Proteins
  • bcl-2-Associated X Protein
  • Cytochromes c