Sensory mechanisms controlling the timing of larval developmental and behavioral transitions require the Drosophila DEG/ENaC subunit, Pickpocket1

Dev Biol. 2008 Oct 1;322(1):46-55. doi: 10.1016/j.ydbio.2008.07.003. Epub 2008 Jul 9.


Growth of multicellular organisms proceeds through a series of precisely timed developmental events requiring coordination between gene expression, behavioral changes, and environmental conditions. In Drosophila melanogaster larvae, the essential midthird instar transition from foraging (feeding) to wandering (non-feeding) behavior occurs prior to pupariation and metamorphosis. The timing of this key transition is coordinated with larval growth and size, but physiological mechanisms regulating this process are poorly understood. Results presented here show that Drosophila larvae associate specific environmental conditions, such as temperature, with food in order to enact appropriate foraging strategies. The transition from foraging to wandering behavior is associated with a striking reversal in the behavioral responses to food-associated stimuli that begins early in the third instar, well before food exit. Genetic manipulations disrupting expression of the Degenerin/Epithelial Sodium Channel subunit, Pickpocket1(PPK1) or function of PPK1 peripheral sensory neurons caused defects in the timing of these behavioral transitions. Transient inactivation experiments demonstrated that sensory input from PPK1 neurons is required during a critical period early in the third instar to influence this developmental transition. Results demonstrate a key role for the PPK1 sensory neurons in regulation of important behavioral transitions associated with developmental progression of larvae from foraging to wandering stage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Behavior, Animal / physiology
  • Chemotaxis / genetics
  • Chemotaxis / physiology
  • Critical Period, Psychological
  • Degenerin Sodium Channels
  • Drosophila Proteins / genetics
  • Drosophila Proteins / physiology*
  • Drosophila melanogaster / growth & development
  • Drosophila melanogaster / physiology*
  • Epithelial Sodium Channels / genetics
  • Epithelial Sodium Channels / physiology*
  • Feeding Behavior / physiology
  • Larva / growth & development
  • Larva / physiology
  • Motor Activity / genetics
  • Motor Activity / physiology
  • Mutation
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / physiology*
  • Protein Subunits / genetics
  • Protein Subunits / physiology
  • Sodium Channels / genetics
  • Sodium Channels / physiology*
  • Temperature


  • Degenerin Sodium Channels
  • Drosophila Proteins
  • Epithelial Sodium Channels
  • Nerve Tissue Proteins
  • Protein Subunits
  • Sodium Channels
  • ppk protein, Drosophila