Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems

V Hinić; I Brodard; A Thomann; Z Cvetnić; P V Makaya; J Frey; C Abril

doi:10.1016/j.mimet.2008.07.002

Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems

J Microbiol Methods. 2008 Oct;75(2):375-8. doi: 10.1016/j.mimet.2008.07.002. Epub 2008 Jul 15.

Authors

V Hinić¹, I Brodard, A Thomann, Z Cvetnić, P V Makaya, J Frey, C Abril

Affiliation

¹ National Centre for Zoonoses, Bacterial Animal Diseases and Antimicrobial Resistance (ZOBA), Institute of Veterinary Bacteriology, University of Bern, Vetsuisse Faculty, Länggass-Strasse 122, P. O. Box, CH-3001 Bern, Switzerland.

PMID: 18675856
DOI: 10.1016/j.mimet.2008.07.002

Abstract

We describe the development of a novel PCR assay for the rapid detection of members of the Brucella genus, and the differentiation between six recognized Brucella species. The assay has proven to be highly specific with the additional advantage of being suitable for use with both conventional and real-time PCR.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacterial Typing Techniques*
Brucella / classification*
Brucella / genetics
Brucella / isolation & purification*
Brucella abortus / classification
Brucella abortus / genetics
Brucella abortus / isolation & purification
Brucella canis / classification
Brucella canis / genetics
Brucella canis / isolation & purification
Brucella melitensis / classification
Brucella melitensis / genetics
Brucella melitensis / isolation & purification
Brucella ovis / classification
Brucella ovis / genetics
Brucella ovis / isolation & purification
Brucella suis / classification
Brucella suis / genetics
Brucella suis / isolation & purification
Brucellosis / microbiology
DNA Primers
DNA, Bacterial / analysis
Dogs
Humans
Polymerase Chain Reaction / methods*
Species Specificity

Substances

DNA Primers
DNA, Bacterial