Abstract
Tiam1 (T-cell lymphoma invasion and metastasis-inducing protein 1), a guanine nucleotide exchange factor that activates Rac, is a colorectal cancer metastasis-related gene. In this study, we aimed to better understand the mechanism underlying Tiam1-mediated metastasis. We applied gene microarray and proteome analysis and compared expression of genes and proteins in a stable Tiam1-silencing colorectal cancer cell line and in a control cell line. Our analysis identified three genes, high-mobility group box1 (HMGB1), annexin IV (ANXA4) and phosphoglycerate mutase 1 (PGAM1) that were associated with Tiam1. Analysis of these proteins, which may be directly or indirectly regulated by Tiam1, may provide insight into the role and mechanism of Tiam1 in colorectal cancer metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Annexin A4 / genetics
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Annexin A4 / metabolism
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Cell Line
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Colorectal Neoplasms / genetics*
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Colorectal Neoplasms / metabolism*
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Colorectal Neoplasms / pathology
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic*
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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HMGB1 Protein / genetics
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HMGB1 Protein / metabolism
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Humans
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Microarray Analysis
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Neoplasm Invasiveness / genetics
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Neoplasm Metastasis / genetics
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Phosphoglycerate Mutase / genetics
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Phosphoglycerate Mutase / metabolism
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Proteome / analysis*
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RNA Interference
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T-Lymphoma Invasion and Metastasis-inducing Protein 1
Substances
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Annexin A4
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Guanine Nucleotide Exchange Factors
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HMGB1 Protein
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Proteome
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T-Lymphoma Invasion and Metastasis-inducing Protein 1
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TIAM1 protein, human
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Phosphoglycerate Mutase