Proportional Change of CD4+CD25+ Regulatory T Cells After Lymphocyte Therapy in Unexplained Recurrent Spontaneous Abortion Patients

Fertil Steril. 2009 Jul;92(1):301-5. doi: 10.1016/j.fertnstert.2008.04.068. Epub 2008 Aug 3.

Abstract

Objective: To investigate the proportional changes of CD4(+)CD25(+) regulatory T cells in peripheral blood after lymphocyte therapy in unexplained recurrent spontaneous abortion (URSA) patients.

Design: Prospective cohort study.

Setting: University Hospital.

Patient(s): Twenty-five URSA patients.

Intervention(s): Measurements of CD4(+)CD25(+) regulatory T cells in peripheral blood before and after paternal or third-party lymphocyte immunization.

Main outcome measure(s): The proportion of CD4(+)CD25(bright) regulatory T cells and the percentage of CD25(bright) cells in the CD4(+) T-cell population.

Result(s): The proportion of CD4(+)CD25(bright) T cells in peripheral blood from URSA patients was increased significantly after paternal or third-party lymphocyte immunization therapy, whereas the percentage of CD4(+)CD25(dim) cells were decreased significantly. The percentage of CD4(+)CD25(bright) cells in the CD4(+) T-cell population was significantly increased, and the proportion of CD4(+)CD25(bright) T cells was significantly higher in successfully pregnant women than in those with pregnancy loss after lymphocyte therapy.

Conclusion(s): Allogeneic lymphocyte therapy can enhance the percentage of CD4(+)CD25(bright) regulatory T cells in peripheral blood, therefore CD4(+)CD25(+) regulatory T cells may serve as a novel biomarker for monitoring allogeneic lymphocyte therapy in URSA patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abortion, Habitual / immunology*
  • Abortion, Habitual / therapy*
  • Abortion, Spontaneous / immunology*
  • Abortion, Spontaneous / therapy*
  • Antigens, CD / immunology
  • Autoantibodies / immunology
  • CD4 Antigens / immunology*
  • Cohort Studies
  • Female
  • Humans
  • Interleukin-2 Receptor alpha Subunit / immunology*
  • Lymphocyte Transfusion*
  • Pregnancy
  • Pregnancy Outcome
  • Prospective Studies
  • T-Lymphocytes, Regulatory / immunology*
  • T-Lymphocytes, Regulatory / transplantation

Substances

  • Antigens, CD
  • Autoantibodies
  • CD4 Antigens
  • Interleukin-2 Receptor alpha Subunit