Caenorhabditis elegans mutant allele identification by whole-genome sequencing

Nat Methods. 2008 Oct;5(10):865-7. doi: 10.1038/nmeth.1249. Epub 2008 Aug 1.


Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Animals
  • Caenorhabditis elegans / genetics*
  • DNA, Helminth / genetics
  • Genome, Helminth*
  • Mutation*
  • Polymorphism, Single Nucleotide


  • DNA, Helminth