Methods for the production of helper-dependent adenoviral vectors

Methods Mol Biol. 2008;433:33-53. doi: 10.1007/978-1-59745-237-3_3.


Helper-dependent adenoviral vectors (HDAd) are deleted of all viral coding sequences and have demonstrated tremendous potential for gene therapy by providing long-term, high-level transgene expression in the absence of chronic toxicity. Thus, HDAd are superior to early generation Ad for gene therapy of genetic diseases where long-term transgene expression is required. This chapter describes in detail the rescue, amplification, and large-scale production of HDAd.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Adenoviridae / isolation & purification
  • Adenoviridae / physiology
  • Cell Count
  • Cell Line
  • Genetic Vectors / biosynthesis*
  • Genetic Vectors / isolation & purification
  • Helper Viruses / metabolism*
  • Humans
  • Integrases / metabolism
  • Molecular Biology / methods*
  • Virus Replication


  • Cre recombinase
  • Integrases