RNA expression analysis from formalin fixed paraffin embedded tissues

Histochem Cell Biol. 2008 Sep;130(3):435-45. doi: 10.1007/s00418-008-0479-7. Epub 2008 Aug 5.


Formalin fixation and paraffin embedding (FFPE) is the most commonly used method worldwide for tissue storage. This method preserves the tissue integrity but causes extensive damage to nucleic acids stored within the tissue. As methods for measuring gene expression such as RT-PCR and microarray are adopted into clinical practice there is an increasing necessity to access the wealth of information locked in the Formalin fixation and paraffin embedding archives. This paper reviews the progress in this field and discusses the unique opportunities that exist for the application of these techniques in the development of personalized medicine.

Publication types

  • Review

MeSH terms

  • Formaldehyde*
  • Gene Expression Regulation / genetics*
  • Humans
  • Paraffin Embedding / methods*
  • RNA / analysis*
  • RNA / genetics*
  • Reproducibility of Results
  • Tissue Fixation / methods*


  • Formaldehyde
  • RNA