Transposon Tn5

Annu Rev Genet. 2008;42:269-86. doi: 10.1146/annurev.genet.42.110807.091656.


Tn5 was one of the first transposons to be identified ( 10 ). As a result of Tn5's early discovery and its simple macromolecular requirements for transposition, the Tn5 system has been a very productive tool for studying the molecular mechanism of DNA transposition. These studies are of broad value because they offer insights into DNA transposition in general, because DNA transposition is a useful model with which to understand other types of protein-DNA interactions such as retroviral DNA integration and the DNA cleavage events involved in immunoglobulin gene formation, and because Tn5-derived tools are useful adjuncts in genetic experimentation.

MeSH terms

  • Bacteria / genetics
  • Bacteria / metabolism
  • Bacteriophage lambda / genetics
  • Base Sequence
  • DNA Transposable Elements / genetics*
  • Drug Resistance, Bacterial / genetics
  • HIV Integrase / chemistry
  • HIV Integrase / genetics
  • HIV Integrase / metabolism
  • Models, Genetic
  • Models, Molecular
  • Molecular Biology
  • Transposases / chemistry
  • Transposases / genetics
  • Transposases / metabolism


  • DNA Transposable Elements
  • HIV Integrase
  • Transposases
  • p31 integrase protein, Human immunodeficiency virus 1