Salidroside attenuates glutamate-induced apoptotic cell death in primary cultured hippocampal neurons of rats

Brain Res. 2008 Oct 31;1238:189-98. doi: 10.1016/j.brainres.2008.07.051. Epub 2008 Jul 22.


Salidroside, a compound of natural origin, has displayed a broad spectrum of pharmacological properties. This study aimed to evaluate the inhibitory effects of salidroside on glutamate-induced cell death in a primary culture of rat hippocampal neurons as compared to brain-derived neurotrophic factor (BDNF), a usual positive control. MTT and LDH assays, together with Hoechst 33342 staining, terminal deoxynucleotidyl transferase dUTP-mediated nicked end labeling (TUNEL) assay and flow cytometric analysis using annexin-V and propidium (PI) label, indicated that salidroside pretreatment attenuated glutamate-induced apoptotic cell death in primary cultured hippocampal neurons, showing a dose-dependent pattern. Furthermore, caspase-3 activity assay and calcium measurements with Fluo 4-AM, respectively, revealed that salidroside pretreatment antagonized activation of caspase-3 and elevation of intracellular calcium level, both of which were induced by glutamate stimulation, thus adding to the understanding of how salidroside offered neuroprotection against glutamate excitotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Blotting, Western
  • Cell Survival / drug effects
  • Cells, Cultured
  • Embryo, Mammalian
  • Flow Cytometry
  • Glucosides / pharmacology*
  • Glutamic Acid / toxicity*
  • Hippocampus / drug effects*
  • Hippocampus / pathology
  • In Situ Nick-End Labeling
  • Neurons / drug effects*
  • Neurons / pathology
  • Neuroprotective Agents / pharmacology*
  • Phenols / pharmacology*
  • Rats
  • Rats, Sprague-Dawley


  • Glucosides
  • Neuroprotective Agents
  • Phenols
  • Glutamic Acid
  • rhodioloside