Chokeberry polyphenols reduce prooxidative influence of oxidized fats in rat diets

Pol J Vet Sci. 2008;11(2):125-32.


The aim of the study was to estimate nutritional and physiological consequences of combined supplementation of rat casein diets with a strongly oxidized mixture of fats and extract of polyphenols from chokeberry. The experiment was conducted on 80 young Wistar rats divided into 8 groups. The animals were fed diets supplemented with 8% of fresh or oxidized fats and 0.4, 0.8 and 1.2% of polyphenol extract together. Addition of the oxidized fats caused significant reduction in animal growth without a decrease in nitrogen digestibility. On the other hand, the extract of polyphenols suppressed nitrogen utilization; animal growth was however similar to the control group. Analysis of the variance showed negative influence of the oxidized fats on cholesterol and triacyloglycerol concentration in the blood of the experimental animals. Increased activity of both aminotransferases in the blood of rats fed diets containing the oxidized fats indicated on liver malfunction. There was no effect of the experimental factors on the antioxidant status of the blood, but a dose-dependent reduced content of malonylodialdehyde in the blood of rats fed diets supplemented with the extract of polyphenols suggested that the extract had a preventive effect against prooxidative activity of the oxidized fats.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animal Feed / standards*
  • Animal Nutritional Physiological Phenomena*
  • Animals
  • Cholesterol / blood
  • Dietary Fats / metabolism
  • Dose-Response Relationship, Drug
  • Flavonoids / pharmacology*
  • Hyperlipidemias / blood
  • Hyperlipidemias / drug therapy
  • Lipid Peroxidation / drug effects*
  • Lipids / blood
  • Male
  • Nitrogen / metabolism
  • Oxidation-Reduction
  • Phenols / pharmacology*
  • Phytotherapy*
  • Plant Extracts / pharmacology
  • Polyphenols
  • Prunus / chemistry*
  • Random Allocation
  • Rats
  • Rats, Wistar / blood
  • Rats, Wistar / growth & development
  • Triglycerides / blood


  • Dietary Fats
  • Flavonoids
  • Lipids
  • Phenols
  • Plant Extracts
  • Polyphenols
  • Triglycerides
  • Cholesterol
  • Nitrogen