Natural variation of HIV-1 group M integrase: implications for a new class of antiretroviral inhibitors

Retrovirology. 2008 Aug 7;5:74. doi: 10.1186/1742-4690-5-74.

Abstract

HIV-1 integrase is the third enzymatic target of antiretroviral (ARV) therapy. However, few data have been published on the distribution of naturally occurring amino acid variation in this enzyme. We therefore characterized the distribution of integrase variants among more than 1,800 published group M HIV-1 isolates from more than 1,500 integrase inhibitor (INI)-naïve individuals. Polymorphism rates equal or above 0.5% were found for 34% of the central core domain positions, 42% of the C-terminal domain positions, and 50% of the N-terminal domain positions. Among 727 ARV-naïve individuals in whom the complete pol gene was sequenced, integrase displayed significantly decreased inter- and intra-subtype diversity and a lower Shannon's entropy than protease or RT. All primary INI-resistance mutations with the exception of E157Q--which was present in 1.1% of sequences--were nonpolymorphic. Several accessory INI-resistance mutations including L74M, T97A, V151I, G163R, and S230N were also polymorphic with polymorphism rates ranging between 0.5% to 2.0%.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Drug Resistance, Viral
  • Genetic Variation*
  • HIV Infections / drug therapy*
  • HIV Infections / veterinary
  • HIV Infections / virology
  • HIV Integrase / chemistry
  • HIV Integrase / genetics*
  • HIV Integrase / metabolism
  • HIV Integrase Inhibitors / pharmacology*
  • HIV Protease / genetics
  • HIV Reverse Transcriptase / genetics
  • HIV-1 / chemistry
  • HIV-1 / drug effects
  • HIV-1 / enzymology*
  • HIV-1 / genetics*
  • Humans
  • Molecular Sequence Data
  • Pan troglodytes
  • Polymorphism, Genetic
  • Protein Structure, Tertiary
  • Sequence Analysis, DNA

Substances

  • HIV Integrase Inhibitors
  • HIV Integrase
  • HIV Reverse Transcriptase
  • HIV Protease