An oxidative stress score as a combined measure of the pro-oxidant and anti-oxidant counterparts in patients with coronary artery disease

Clin Biochem. 2008 Oct;41(14-15):1162-7. doi: 10.1016/j.clinbiochem.2008.07.005. Epub 2008 Jul 26.


Oxidative stress plays a key role in the pathogenesis and development of atherosclerosis.

Aim: To evaluate the relationship between a novel oxidative stress index (reflecting both oxidative and anti-oxidant counterparts) with traditional cardiovascular risk factors and C-reactive protein (CRP) in coronary artery disease (CAD).

Methods: 100 angiographically proven CAD and 70 control subjects (mean age: 65+/-10 years, 110 males), underwent a global cardiovascular risk assessment and serum CRP and oxidative stress estimation. The Oxidative-INDEX was calculated after automated evaluation of serum hydroperoxides and total anti-oxidant capacity (D-ROM and OXY-adsorbent Test, Diacron, Italy) subtracting the OXY standardized variable from the ROM standardized variable.

Results: The Oxidative-INDEX was higher in CAD with respect to control subjects (p < 0.001). A stepwise elevation in the Oxidative-INDEX levels was found depending on the number of affected vessels (p < 0.001). Oxidative stress was elevated according to the presence of diabetes (p < 0.001), smoking habit (p < 0.01), and hypercholesterolemia (p < 0.05). Oxidative-INDEX significantly correlated with aging (p < or = 0.05) and CRP (p < 0.001). The Oxidative-INDEX increased with the number of cardiovascular risk factors (p < 0.001). After adjustment for traditional CV risk factors, the multivariate logistic regression analysis indicated the Oxidative-INDEX concentration as an independent factor for CAD (odds ratio=1.4, confidence intervals=1.1-1.9, p < 0.05).

Conclusion: Oxidative stress represents a shared molecular pathway in atherosclerotic-related conditions, and its estimation by the automated Oxidative-INDEX could represent a valuable tool and a promising target in the prevention, diagnosis and treatment of CAD in the clinical setting.

MeSH terms

  • Aged
  • Antioxidants / metabolism*
  • Atherosclerosis / blood
  • Atherosclerosis / complications
  • Case-Control Studies
  • Coronary Artery Disease / blood*
  • Coronary Artery Disease / complications
  • Demography
  • Female
  • Humans
  • Male
  • Multivariate Analysis
  • Oxidative Stress*
  • Reactive Oxygen Species / blood*
  • Risk Factors


  • Antioxidants
  • Reactive Oxygen Species