A semi-dominant mutation in the ribosomal protein L10 gene suppresses the dwarf phenotype of the acl5 mutant in Arabidopsis thaliana

Plant J. 2008 Dec;56(6):881-90. doi: 10.1111/j.1365-313X.2008.03647.x. Epub 2008 Aug 6.


Disruption of the Arabidopsis thaliana ACAULIS5 (ACL5) gene, which has recently been shown to encode thermospermine synthase, results in a severe dwarf phenotype. A previous study showed that sac51-d, a dominant suppressor mutant of acl5-1, has a premature termination codon in an upstream open reading frame (ORF) of SAC51, which encodes a putative transcription factor, and suggested the involvement of upstream ORF-mediated translational control in ACL5-dependent stem elongation. Here we report the identification of a gene responsible for sac52-d, another semi-dominant suppressor mutant of acl5-1. SAC52 encodes ribosomal protein L10 (RPL10A), which is highly conserved among eukaryotes and implicated in translational regulation. Transformation of acl5-1 mutants with a genomic fragment containing the sac52-d allele rescued the dwarf phenotype of acl5-1. GUS reporter activity under the control of a SAC51 promoter with its upstream ORF was higher in acl5-1 sac52-d than in acl5-1, suggesting that suppression of the acl5-1 phenotype by sac52-d is attributable, in part, to enhanced translation of certain transcripts including SAC51. We also found that a T-DNA insertion allele of SAC52/RPL10A causes lethality in the female gametophyte.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Arabidopsis / genetics*
  • Arabidopsis / growth & development
  • Arabidopsis / metabolism
  • Arabidopsis Proteins / genetics*
  • Arabidopsis Proteins / metabolism
  • Cloning, Molecular
  • DNA, Bacterial / genetics
  • DNA, Plant / genetics
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Mutation
  • Phenotype
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Ribosomal Protein L10
  • Ribosomal Proteins / genetics*
  • Ribosomal Proteins / metabolism
  • Sequence Alignment


  • ACAULIS5 protein, Arabidopsis
  • Arabidopsis Proteins
  • DNA, Bacterial
  • DNA, Plant
  • RPL10A protein, Arabidopsis
  • Ribosomal Proteins
  • T-DNA
  • ribosomal protein L10, Arabidopsis