The dynamics of the nitric oxide release-transient from stretched muscle cells

Int J Biochem Cell Biol. 2009 Mar;41(3):625-31. doi: 10.1016/j.biocel.2008.07.005. Epub 2008 Jul 25.


Potent nitric oxide (NO) signals are described for many forms of cell-cell communication. Although NO plays a significant role in skeletal muscle metabolism and contractility and in precursor activation during muscle formation and stretching, there is no direct evidence of stretch-induced NO release from muscle. Differentiated muscle cell cultures from normal and dystrophic mdx mice were preloaded with the NO-specific dye DAF-2 (diaminofluorescein-2) before stretching. NO release was detected by video-microscopy. NO was released rapidly from wild-type (WT) cells after stretch and intensity declined rapidly to a plateau. Mdx cells showed much less NO release. Direct observations of the time-course of stretch-induced NO release in WT cells is congruent with the hypothesis of NO-mediated stretch activation of satellite cells in normal skeletal muscle. Distinct differences in the time-course between normal and dystrophic cells indicate visualization methods for NO release will be a sensitive measure of NOS-1 restoration following diverse treatment approaches to muscular dystrophy.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Dystrophin / genetics
  • Dystrophin / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred mdx
  • Molecular Diagnostic Techniques
  • Muscular Dystrophy, Duchenne / diagnosis*
  • Muscular Dystrophy, Duchenne / pathology
  • Nitric Oxide / biosynthesis
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase Type I / genetics
  • Nitric Oxide Synthase Type I / metabolism*
  • Satellite Cells, Skeletal Muscle / metabolism*
  • Satellite Cells, Skeletal Muscle / pathology
  • Stem Cells / pathology
  • Stress, Mechanical
  • Time Factors


  • Dystrophin
  • Nitric Oxide
  • Nitric Oxide Synthase Type I
  • Nos1 protein, mouse