Protein modification for single molecule fluorescence microscopy

Org Biomol Chem. 2008 Sep 7;6(17):3031-7. doi: 10.1039/b808552h. Epub 2008 Jul 14.


Single molecule methods have emerged as a powerful new tool for exploring biological phenomena. We provide a brief overview of the scope of current experiments and assess the limitations of both fluorescent labels and the means to achieve protein modification for single molecule microscopy.

Publication types

  • Review

MeSH terms

  • Fluorescent Dyes*
  • Green Fluorescent Proteins*
  • Microscopy, Fluorescence*
  • Molecular Probes*
  • Nanotechnology*
  • Protein Processing, Post-Translational*
  • Proteins / chemistry*


  • Fluorescent Dyes
  • Molecular Probes
  • Proteins
  • Green Fluorescent Proteins