Enhanced levels of prostaglandin E2 and matrix metalloproteinase-2 correlate with the severity of airflow limitation in stable COPD

Respirology. 2008 Nov;13(7):1014-21. doi: 10.1111/j.1440-1843.2008.01365.x.


Background and objective: Cyclooxygenase-2 (COX-2) and its product prostaglandin E2 (PGE2) have been demonstrated to play critical roles in inflammation in respiratory diseases. However, the role of COX-2 in airway remodelling in COPD remains to be elucidated. Matrix metalloproteinase-2 (MMP-2) is associated with both inflammation and airway remodelling in COPD. The objective of this study was to measure the expression of COX-2 and the concentrations of PGE2 and MMP-2, and to investigate the role of COX-2 and PGE2 in airflow limitation mediated by MMP-2, in the pathogenesis of COPD.

Methods: Forty-three patients with stable COPD, twelve smoking control subjects and ten nonsmoking control subjects were enrolled. Induced sputum was obtained for measurement of the concentrations of PGE2 and MMP-2 by ELISA. COX-2 protein expression was assessed by western blotting.

Results: PGE2 and MMP-2 concentrations were significantly higher in both smoking control subjects and patients with COPD than in non-smoking control subjects (P < 0.01).Moreover, the levels of PGE2 andMMP-2 were inversely correlated with FEV1%predicted in COPD patients (PGE2: r = -0.748, P < 0.01; MMP-2: r = -0.801, P < 0.01). Levels of PGE2 were also positively correlated with those of MMP-2 in patients with COPD (r = 0.775, P < 0.01). Expression of COX-2 protein was significantly higher in COPD patients than in non-smoking control subjects.

Conclusions: COX-2 and its product PGE2 are not only involved in airway inflammation, but may also contribute to the severity of airflow limitation mediated by MMP-2 during progression of COPD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Airway Resistance / physiology*
  • Asthma / diagnosis
  • Asthma / metabolism*
  • Asthma / physiopathology
  • Blotting, Western
  • Cyclooxygenase 2 / biosynthesis
  • Dinoprostone / biosynthesis*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Follow-Up Studies
  • Forced Expiratory Volume / physiology
  • Humans
  • Male
  • Matrix Metalloproteinase 2 / biosynthesis*
  • Middle Aged
  • Severity of Illness Index
  • Sputum / chemistry*
  • Time Factors


  • Cyclooxygenase 2
  • Matrix Metalloproteinase 2
  • Dinoprostone