Substrate profiling of PRMT1 reveals amino acid sequences that extend beyond the "RGG" paradigm

Biochemistry. 2008 Sep 9;47(36):9456-66. doi: 10.1021/bi800984s. Epub 2008 Aug 13.

Abstract

Protein arginine methyltransferase 1 (PRMT1) catalyzes the mono- and dimethylation of certain protein arginine residues. Although this posttranslational modification has been implicated in many physiological processes, the molecular basis for PRMT1 substrate recognition is poorly understood. Most modified arginine residues in known PRMT1 substrates reside in repeating "RGG" sequences. However, PRMT1 also specifically methylates Arg3 of histone H4 in a region that is not glycine-arginine rich, suggesting that PRMT1 substrates are not limited to proteins bearing "RGG" sequences. Because a systematic evaluation of PRMT1 substrate specificity has not been performed, it is unclear if the "RGG" sequence accurately represents the consensus target for PRMT1. Using a focused peptide library based on a sequence derived from the in vivo substrate fibrillarin we observed that PRMT1 methylated substrates that had amino acid residues other than glycine in the "RX (1)" and "RX (1)X (2)" positions. Importantly, eleven additional PRMT1 substrate sequences were identified. Our results also illustrate that the two residues on the N-terminal side of the modification site are important and need not both be glycine. PRMT1 methylated the eukaryotic initiation factor 4A1 (eIF4A1) protein, which has a single "RGG" sequence. Methylation of eIF4A1 and the similar eIF4A3 could be affected using single site mutations adjacent to the modification site, demonstrating the importance of amino acid sequence in PRMT1 protein substrates. Dimethylation of the parent library peptide was shown to occur through a dissociative mechanism. In summary, PRMT1 selectively recognizes a set of amino acid sequences in substrates that extend beyond the "RGG" paradigm.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Motifs / physiology
  • Animals
  • Arginine / chemistry*
  • Arginine / metabolism
  • Chromosomal Proteins, Non-Histone / chemistry
  • Chromosomal Proteins, Non-Histone / metabolism
  • Eukaryotic Initiation Factor-4A / chemistry
  • Eukaryotic Initiation Factor-4A / metabolism
  • Histones / chemistry
  • Histones / metabolism
  • Humans
  • Methylation
  • Peptide Library
  • Peptides / chemistry*
  • Peptides / metabolism
  • Protein Processing, Post-Translational / physiology
  • Protein Structure, Tertiary / physiology
  • Protein-Arginine N-Methyltransferases / chemistry*
  • Protein-Arginine N-Methyltransferases / metabolism
  • Repressor Proteins / chemistry*
  • Repressor Proteins / metabolism
  • Substrate Specificity / physiology

Substances

  • Chromosomal Proteins, Non-Histone
  • Histones
  • Peptide Library
  • Peptides
  • Repressor Proteins
  • fibrillarin
  • Arginine
  • PRMT1 protein, human
  • Protein-Arginine N-Methyltransferases
  • Eukaryotic Initiation Factor-4A