Chromophore-assisted laser inactivation in cell biology

Trends Cell Biol. 2008 Sep;18(9):443-50. doi: 10.1016/j.tcb.2008.07.001. Epub 2008 Aug 14.


Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. CALI is an important complement to conventional genetic and pharmacological manipulations. In this review, we examine the applications of CALI to cell biology and discuss the underlying photochemical mechanisms that mediate this powerful technique.

Publication types

  • Research Support, N.I.H., Extramural
  • Review

MeSH terms

  • Animals
  • Cell Physiological Phenomena*
  • Fluorescent Dyes
  • Green Fluorescent Proteins / chemistry
  • Humans
  • Lasers*
  • Photobleaching
  • Photochemical Processes*
  • Photosensitizing Agents / chemistry*
  • Reactive Oxygen Species / chemistry*
  • Singlet Oxygen / chemistry


  • Fluorescent Dyes
  • Photosensitizing Agents
  • Reactive Oxygen Species
  • Green Fluorescent Proteins
  • Singlet Oxygen