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. 2008 Oct 24;375(3):346-50.
doi: 10.1016/j.bbrc.2008.08.020. Epub 2008 Aug 14.

Hydrogen-rich Pure Water Prevents Superoxide Formation in Brain Slices of Vitamin C-depleted SMP30/GNL Knockout Mice

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Hydrogen-rich Pure Water Prevents Superoxide Formation in Brain Slices of Vitamin C-depleted SMP30/GNL Knockout Mice

Yasunori Sato et al. Biochem Biophys Res Commun. .

Abstract

Hydrogen is an established anti-oxidant that prevents acute oxidative stress. To clarify the mechanism of hydrogen's effect in the brain, we administered hydrogen-rich pure water (H(2)) to senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize vitamin C (VC), also a well-known anti-oxidant. These KO mice were divided into three groups; recipients of H(2), VC, or pure water (H(2)O), administered for 33 days. VC levels in H(2) and H(2)O groups were <6% of those in the VC group. Subsequently, superoxide formation during hypoxia-reoxygenation treatment of brain slices from these groups was estimated by a real-time biography imaging system, which models living brain tissues, with Lucigenin used as chemiluminescence probe for superoxide. A significant 27.2% less superoxide formed in the H(2) group subjected to ischemia-reperfusion than in the H(2)O group. Thus hydrogen-rich pure water acts as an anti-oxidant in the brain slices and prevents superoxide formation.

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