CD5-low expression lymphocytes in canine peripheral blood show characteristics of natural killer cells

J Leukoc Biol. 2008 Dec;84(6):1501-10. doi: 10.1189/jlb.0408255. Epub 2008 Aug 15.


NK cell markers and receptors have been discovered in many mammalian species, such as humans, mice, rats, pigs, and cows. However, there is still a lack of information concerning NK cell markers or receptors in canines. We have discovered that canine CD5-low density (CD5lo) cells in PBL are closely associated with NK cell characteristics. CD5lo cells comprised 14.9 +/- 6.68% of the total PBL. A high proportion of the CD5lo cell population expressed CD3 (96.6%), CD8alpha (77.7%), CD8beta (53%), alpha/beta TCR (83%), and CD11/18 (80%), but the expression of gamma/delta TCR (6.5%), CD4 (10.6%), and CD21 (2.4%) was low. CD5lo cells were larger than CD5-high density (CD5hi) cells. Light and electron microscopy revealed numerous large cytoplasmic granules in CD5lo cells, especially after IL-2 stimulation, which was in contrast to CD5hi, in which intracytoplasmic granules were not frequently seen. After IL-2 stimulation, CD5lo cells had significantly stronger NK cytotoxicity than CD5hi cells. CD5lo cells had much higher mRNA levels for NKG2D, CD16, CD94, CD160, perforin, and granzyme than CD5hi. Following IL-2 stimulation, CD5lo cells had significantly higher mRNA levels of NKp30, NKp44, CD16, and CD94 than CD5hi cells. In addition, IL-2-stimulated, CD5lo-depleted PBL showed a loss of NK cytotoxicity. CD5lo cells also showed significantly lower antigen-specific cytotoxic T cell activity as compared with CD5hi cells. Taken together, the CD5lo subset in canine PBL is closely related to canine NK cells, and CD5lo can be used as a phenotypic marker for an IL-2-dependent canine NK cell enrichment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD5 Antigens / metabolism*
  • Cytotoxicity Tests, Immunologic
  • Dogs
  • Female
  • Flow Cytometry
  • Gene Expression
  • Interferon-gamma / metabolism
  • Interleukin-15 / metabolism
  • Interleukin-18 / metabolism
  • Killer Cells, Lymphokine-Activated / immunology*
  • Leukocytes / metabolism
  • Lymphocyte Subsets*
  • Phenotype
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism*
  • T-Lymphocytes, Cytotoxic / immunology


  • CD5 Antigens
  • Interleukin-15
  • Interleukin-18
  • RNA, Messenger
  • Interferon-gamma