Cryopreservation has no effect on meiotic recombination and synapsis in testicular tissues

Jianhua Li; Mei Leng; Tieliang Ma; Dexin Yu; Haoqiang Shi; Qinghua Shi

doi:10.1016/j.fertnstert.2008.04.074

Cryopreservation has no effect on meiotic recombination and synapsis in testicular tissues

Fertil Steril. 2009 Apr;91(4 Suppl):1404-7. doi: 10.1016/j.fertnstert.2008.04.074. Epub 2008 Aug 16.

Authors

Jianhua Li¹, Mei Leng, Tieliang Ma, Dexin Yu, Haoqiang Shi, Qinghua Shi

Affiliation

¹ Laboratory of Molecular and Cell Genetics, Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei, Anhui, People's Republic of China.

PMID: 18710708
DOI: 10.1016/j.fertnstert.2008.04.074

Abstract

The effects of cryopreservation on meiotic progression, synapsis, recombination, and structure of synaptonemal complexes (SCs) in testicular tissues were evaluated by comparing the above-mentioned parameters in frozen and fresh testicular tissues from the same men. No differences in meiotic progression, the mean number of MLH1 foci per cell, the mean number of autosomal SCs with different numbers of MLH1 foci, or the fidelity of the synapsis were observed between fresh and frozen testicular tissues.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / metabolism
Adult
Aged
Chromosome Pairing / physiology*
Cryopreservation / methods*
Humans
Male
Meiosis / physiology*
Middle Aged
MutL Protein Homolog 1
Nuclear Proteins / metabolism
Spermatocytes / cytology
Spermatocytes / metabolism
Synaptonemal Complex / physiology
Testis / cytology*
Testis / metabolism

Substances

Adaptor Proteins, Signal Transducing
MLH1 protein, human
Nuclear Proteins
MutL Protein Homolog 1