Plasma NEFA storage in adipose tissue in the postprandial state: sex-related and regional differences

Diabetologia. 2008 Nov;51(11):2041-8. doi: 10.1007/s00125-008-1126-5. Epub 2008 Aug 19.


Aims/hypothesis: We recently reported that a small fraction of circulating NEFA is stored through direct uptake in subcutaneous fat in postabsorptive humans in vivo and that this pathway may favour lower-body fat distribution in women. Here, we examined sex-related and regional differences in storage of plasma NEFA in subcutaneous adipose tissue during postprandial conditions.

Methods: At 1 h after lunch, men and women of normal weight received an intravenous bolus of approximately 1.66 MBq [1-(14)C]oleate followed by timed subcutaneous fat biopsies. The preceding breakfast was either a normal- or high-fat meal; the high-fat breakfast was used to create postprandial oleate concentrations in the postabsorptive range.

Results: Storage of the NEFA tracer in adipose tissue (dpm/g lipid) was greater in women; in both sexes abdominal fat stored tracer more avidly than femoral fat. A greater fraction of the administered tracer was stored in whole body subcutaneous fat of women than in that of men (27 +/- 3 vs 8 +/- 1%, respectively, p < 0.0001). No significant differences in tracer storage were observed between participants consuming the high- vs normal-fat breakfast.

Conclusions/interpretation: Postprandial NEFA storage in subcutaneous fat through direct uptake accounts for approximately 25% of NEFA disposal in women, but for <10% in men in a wide range of circulating NEFA concentrations. It is greater in the upper- than lower-body subcutaneous fat, favouring upper-body fat accumulation in both sexes.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Adult
  • Blood Glucose / metabolism
  • Energy Intake
  • Fatty Acids, Nonesterified / blood
  • Fatty Acids, Nonesterified / metabolism*
  • Female
  • Humans
  • Insulin / blood
  • Lipoproteins, VLDL / metabolism
  • Male
  • Postprandial Period*
  • Premenopause
  • Reference Values
  • Sex Characteristics


  • Blood Glucose
  • Fatty Acids, Nonesterified
  • Insulin
  • Lipoproteins, VLDL