Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains

J Clin Microbiol. 2008 Oct;46(10):3484-7. doi: 10.1128/JCM.00837-08. Epub 2008 Aug 20.

Abstract

An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.

Publication types

  • Evaluation Study
  • Multicenter Study

MeSH terms

  • Animals
  • Bacterial Typing Techniques*
  • Brucella / classification*
  • Brucella / genetics*
  • DNA Primers / genetics
  • Humans
  • Mammals
  • Polymerase Chain Reaction / methods*

Substances

  • DNA Primers