Ecofriendly biodegradation and detoxification of Reactive Red 2 textile dye by newly isolated Pseudomonas sp. SUK1

J Hazard Mater. 2009 Apr 30;163(2-3):735-42. doi: 10.1016/j.jhazmat.2008.07.020. Epub 2008 Jul 12.

Abstract

The aim of this work is to evaluate textile dyes degradation by novel bacterial strain isolated from the waste disposal sites of local textile industries. Detailed taxonomic studies identified the organisms as Pseudomonas species and designated as strain Pseudomonas sp. SUK1. The isolate was able to decolorize sulfonated azo dye (Reactive Red 2) in a wide range (up to 5 g l(-1)), at temperature 30 degrees C, and pH range 6.2-7.5 in static condition. This isolate also showed decolorization of the media containing a mixture of dyes. Measurements of COD were done at regular intervals to have an idea of mineralization, showing 52% reduction in the COD within 24h. Induction in the activity of lignin peroxidase and azoreductase was observed during decolorization of Reactive Red 2 in the batch culture, which represented their role in degradation. The biodegradation was monitored by UV-vis, IR spectroscopy, HPLC. The final product, 2-naphthol was characterized by GC-mass spectroscopy. The phytotoxicity study revealed the degradation of Reactive Red 2 into non-toxic product by Pseudomonas sp. SUK1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics
  • Biodegradation, Environmental*
  • Industrial Waste*
  • NADH, NADPH Oxidoreductases / genetics
  • Naphthalenesulfonates / metabolism*
  • Peroxidases / genetics
  • Pseudomonas / enzymology
  • Pseudomonas / metabolism*
  • Textile Industry
  • Transcriptional Activation
  • Triazines / metabolism*

Substances

  • Bacterial Proteins
  • Industrial Waste
  • Naphthalenesulfonates
  • Triazines
  • reactive red 2
  • Peroxidases
  • lignin peroxidase
  • NADH, NADPH Oxidoreductases
  • azoreductase