Nerve growth factor and brain-derived neurotrophic factor attenuate angiotensin-II-induced facilitation of calcium channels in acutely dissociated nucleus tractus solitarii neurons of the rat

Arch Oral Biol. 2008 Dec;53(12):1192-201. doi: 10.1016/j.archoralbio.2008.07.006. Epub 2008 Aug 23.


Objective: Neurotrophins, such as nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), promote neuronal development and neuronal survival, but their mechanisms remain controversial. This study aimed to investigate the hypothesis that NGF and BDNF interfere with angiotensin-II- and glutamate-induced facilitation of voltage-dependent Ca(2+) channels (VDCCs) in nucleus tractus solitarius (NTS) neurons.

Design: The profile of NGF and BDNF actions in acutely dissociated rat NTS was studied using the whole-cell configuration of the patch-clamp technique.

Results: Pretreatment with NGF and BDNF attenuated angiotensin-II-induced facilitation of VDCCs, but did not attenuate glutamate-induced facilitation of the L-type VDCC current in NTS neurons. NGF-induced attenuation was antagonised by pretreatment with a tyrosine kinase A (TrkA) receptor antagonist K-252a.

Conclusions: NGF attenuated angiotensin-II-induced facilitation of L-type VDCCs mediated by TrkA receptors in NTS neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin II / pharmacology*
  • Animals
  • Brain-Derived Neurotrophic Factor / pharmacology*
  • Brain-Derived Neurotrophic Factor / physiology
  • Calcium Channels / drug effects*
  • Calcium Channels / physiology
  • Calcium Signaling / drug effects
  • Calcium Signaling / physiology
  • Nerve Growth Factor / pharmacology*
  • Nerve Growth Factor / physiology
  • Patch-Clamp Techniques
  • Rats
  • Rats, Wistar
  • Solitary Nucleus / drug effects*
  • Solitary Nucleus / physiology
  • Vasoconstrictor Agents / pharmacology*


  • Brain-Derived Neurotrophic Factor
  • Calcium Channels
  • Vasoconstrictor Agents
  • Angiotensin II
  • Nerve Growth Factor