Human herpesvirus 8 DNA quantification in matched plasma and PBMCs samples of patients with HHV8-related lymphoproliferative diseases

J Clin Virol. 2008 Nov;43(3):255-9. doi: 10.1016/j.jcv.2008.07.005. Epub 2008 Aug 23.


Background: The quantitative evaluation of human herpesvirus 8 (HHV8) DNA is not well described in the clinical management of HHV8-related lymphoproliferative diseases.

Objectives: To evaluate and to compare HHV8 viral load in different blood compartments from patients with multicentric Castleman's disease (MCD), primary effusion lymphoma (PEL) and HHV8-associated solid lymphoma (SLY) and to establish which clinical sample would be preferable for HHV8 DNA testing.

Study design: We assessed HHV8 DNA in plasma and peripheral blood mononuclear cells (PBMCs) paired samples from 7 PEL, 8 MCD, 2 SLY HIV+ patients at the diagnosis and during the course of the illness by using a real time PCR assay.

Results: HHV8 viremia was always detectable at diagnosis. HHV8 DNA levels were correlated in matched pairs of samples at diagnosis and during follow-up (Spearman correlation coefficient: r=0.83, p<0.001 and r=0.73, p<0.001, respectively). The performance characteristics of the PCR assay with both materials did not show disparity by the analysis of the receiver operating characteristic (ROC) curve (X(1)(2)=0.50; p=0.48).

Conclusions: Plasma or PBMCs are both adequate samples for HHV8 DNA quantification and Real time PCR provides a reliable method to estimate viral replication in patients with HHV8-related lymphoproliferations, where HHV8 viral load is a consistent feature.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Castleman Disease / virology
  • DNA, Viral / blood*
  • Herpesviridae Infections / virology*
  • Herpesvirus 8, Human / isolation & purification*
  • Humans
  • Leukocytes, Mononuclear / virology*
  • Lymphoma / virology
  • Lymphoma, Primary Effusion / virology
  • Plasma / virology*
  • Polymerase Chain Reaction / methods
  • ROC Curve
  • Viral Load*


  • DNA, Viral