A starvation-induced noncoding RNA modulates expression of Dicer-regulated genes

Proc Natl Acad Sci U S A. 2008 Sep 2;105(35):12897-902. doi: 10.1073/pnas.0805118105. Epub 2008 Aug 22.

Abstract

Although much has been learned about short noncoding RNAs, long noncoding transcripts are largely uncharacterized. Here, we describe Caenorhabditis elegans rncs-1, a highly base-paired, 800-nucleotide noncoding RNA expressed in hypodermis and intestine. Transcription of rncs-1 is modulated in response to food supply. Although highly double-stranded, we show that rncs-1 RNA is not a substrate for Dicer because of branched structures at its termini. However, rncs-1 RNA inhibits Dicer cleavage of a second dsRNA in vitro, presumably by competition. We validate this observation in vivo by demonstrating that mRNA levels of several Dicer-regulated genes vary with changes in rncs-1 expression. Certain viruses express dsRNA to compete with cellular dsRNA-mediated pathways, and our data suggest that rncs-1 provides a cellular correlate of this phenomenon.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / enzymology*
  • Caenorhabditis elegans / genetics*
  • Food Deprivation*
  • Gene Expression Regulation*
  • Genes, Helminth
  • Genome
  • Intestinal Mucosa / metabolism
  • RNA Processing, Post-Transcriptional
  • RNA, Small Interfering / metabolism
  • RNA, Untranslated / genetics*
  • Ribonuclease III / metabolism*
  • Subcutaneous Tissue / metabolism
  • Transcription, Genetic

Substances

  • RNA, Small Interfering
  • RNA, Untranslated
  • Ribonuclease III