Embryonic stem cells derived from C57BL/6J and C57BL/6N mice

Comp Med. 2008 Aug;58(4):347-52.


Mouse embryonic stem (ES) cells with the C57BL/6 genetic background allow the generation of knockout mice without the need to backcross to C57BL/6. However, C57BL/6 ES cells whose pluripotency after homologous recombination has been confirmed are not yet available from public cell banks. To facilitate the use of ES cells derived from C57BL/6 sublines in both biologic and medical research, we demonstrated that the use of knockout serum replacement as a medium supplement and 8-cell blastomeres as recipient embryos allowed establishment of ES cells and production of germline chimeric mice, respectively. Under effective conditions, a large number of ES cell lines were established from C57BL/6J and C57BL/6N blastocysts. The majority of ES cells in many cell lines obtained from both strains showed a normal chromosome number. Germline chimeric mice were generated from C57BL/6J and C57BL/6N ES cells. Finally, the ES cell line B6J-S1UTR, derived from C57BL/6J, was used for successful production of gene knockout mice. C57BL/6J ES (B6J-S1UTR and B6J-23UTR) and C57BL/6N ES (B6N-22UTR) cells are available from the cell bank of the BioResource Center at RIKEN Tsukuba Institute (http://www.brc.riken.jp/lab/cell/english/).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques
  • Cell Line
  • Cell Shape
  • Cells, Cultured
  • Chimera / genetics
  • Coculture Techniques
  • Culture Media / chemistry
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / physiology*
  • Gene Targeting
  • Humans
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL*
  • Mice, Knockout
  • Mice, Transgenic
  • Recombination, Genetic


  • Culture Media