An endonuclease activity in human polymorphonuclear neutrophils that removes 8-hydroxyguanine residues from DNA+

Biochem Biophys Res Commun. 1991 Aug 15;178(3):1472-8. doi: 10.1016/0006-291x(91)91059-l.

Abstract

An endonuclease that specifically removes 8-hydroxyguanine (oh8Gua) from DNA has been isolated from Escherichia coli. As the amount of oh8Gua produced in DNA of X-ray-irradiated mice is known to decrease with time after irradiation, an attempt was made to find a similar activity in human polymorphonuclear neutrophils (PMNs) using a synthetic dsDNA containing oh8Gua as a substrate. The PMN enzyme was isolated free of other DNases, and found to cleave the substrate DNA simultaneously at 2 sites, the phosphodiester bonds 5' and 3' to oh8Gua, producing free hydroxyl and phosphate groups, respectively. The enzyme showed almost no activity on DNAs containing other kinds of modified base tested or mismatched DNA. Thus human cells also contain an endonuclease that specifically removes oh8Gua residues from DNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Damage*
  • DNA, Single-Stranded / metabolism*
  • DNA-Formamidopyrimidine Glycosylase
  • Endodeoxyribonucleases / blood*
  • Endodeoxyribonucleases / isolation & purification
  • Escherichia coli Proteins*
  • Guanine / analogs & derivatives*
  • Humans
  • Male
  • Molecular Sequence Data
  • N-Glycosyl Hydrolases / blood
  • N-Glycosyl Hydrolases / isolation & purification
  • Neutrophils / enzymology*
  • Substrate Specificity

Substances

  • DNA, Single-Stranded
  • Escherichia coli Proteins
  • 8-hydroxyguanine
  • Guanine
  • Endodeoxyribonucleases
  • N-Glycosyl Hydrolases
  • DNA-Formamidopyrimidine Glycosylase
  • DNA-formamidopyrimidine glycosylase, E coli