Gene cloning and nucleotide sequence of SM22 alpha from the chicken gizzard smooth muscle

Biochem Int. 1991 Mar;23(4):663-8.

Abstract

A full length of cDNA encoding SM22 alpha from chicken gizzard smooth muscle was cloned and sequenced. Cloned cDNA had a total length of 1214bp and contained a single open reading frame which encodes 200 amino acids with a calculated molecular weight 22214. The predicted amino acid sequence was in complete agreement with the sequence determined by Pearlstone et al. using Edman degradation method (J. Biol. Chem. 262:5985, 1987) except for two additional residues, isoleucine and serine at the C-terminus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Chickens / genetics*
  • Cloning, Molecular
  • Gizzard, Avian / metabolism
  • Microfilament Proteins*
  • Molecular Sequence Data
  • Muscle Proteins / genetics*
  • Muscle, Smooth / metabolism
  • Open Reading Frames / genetics

Substances

  • Microfilament Proteins
  • Muscle Proteins
  • Tagln protein, mouse
  • transgelin

Associated data

  • GENBANK/M64055
  • GENBANK/M64056
  • GENBANK/M64057
  • GENBANK/M64058
  • GENBANK/M64059
  • GENBANK/M64060
  • GENBANK/M83105
  • GENBANK/M83106
  • GENBANK/M83107
  • GENBANK/S67100