Enhancer detection and developmental expression of zebrafish sprouty1, a member of the fgf8 synexpression group

Dev Dyn. 2008 Sep;237(9):2594-603. doi: 10.1002/dvdy.21689.


Signaling pathways mediated by receptor tyrosine kinases (RTKs) are under positive and negative regulation, and misregulation of RTK signaling results in developmental defects and malignancy. A major class of antagonists of Fgf and Egf signaling are the Sprouty proteins. Through an enhancer detection approach, we isolated the sprouty1 (spry1) gene, expressed in multiple developing organs during embryogenesis. We analyzed expression of spry1 between tail bud stage and 10 days postfertilization. From the tail bud stage on, transcript and reporter are detected in the craniofacial region and in the mid-hindbrain boundary, where expression persists until adulthood. Further expression domains are the telencephalon, hindbrain, dorsal diencephalon and epiphysis, branchial arches, pituitary, and the tubular gill epithelium. In the trunk spry1 is also prominently expressed in pronephros, the lateral line and tail fin. Sprouty1 acts in Fgf signaling downstream of Fgfr1, as its expression is abrogated through the small molecule inhibitor of this receptor, SU5402.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Fibroblast Growth Factors / chemistry
  • Fibroblast Growth Factors / classification
  • Fibroblast Growth Factors / genetics*
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Molecular Sequence Data
  • Nervous System / drug effects
  • Nervous System / embryology
  • Nervous System / metabolism
  • Phylogeny
  • Pyrroles / pharmacology
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Zebrafish / embryology
  • Zebrafish / genetics*
  • Zebrafish Proteins / chemistry
  • Zebrafish Proteins / classification
  • Zebrafish Proteins / genetics*


  • Pyrroles
  • SU 5402
  • Zebrafish Proteins
  • fgf8a protein, zebrafish
  • Fibroblast Growth Factors