Granulocyte/macrophage colony-stimulating factor and interleukin 3 release from human peripheral blood eosinophils and neutrophils

J Exp Med. 1991 Sep 1;174(3):745-8. doi: 10.1084/jem.174.3.745.


Human peripheral blood eosinophils released eosinophil survival-enhancing activity when stimulated with the calcium ionophore, ionomycin. The release of activity was detected as early as 3 h after stimulation and was inhibited by an immunomodulating agent, cyclosporin A. The survival-enhancing activity was completely abolished by treatment with anti-interleukin 3 (IL-3) and anti-granulocyte/macrophage colony-stimulating factor (GM-CSF) monoclonal antibodies. Moreover, IL-3 and GM-CSF were measurable in ionomycin-stimulated eosinophil supernatants by immunoassay. Eosinophils produced approximately one-half as much IL-3 and one-fifth as much GM-CSF as ionomycin-stimulated mononuclear cells. Neutrophils also produced IL-3 and GM-CSF, but the amounts were less than those produced by eosinophils. These observations suggest a novel role for eosinophils in pathophysiology of allergic inflammation and host defense mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Survival / drug effects
  • Eosinophil-Derived Neurotoxin
  • Eosinophils / cytology
  • Eosinophils / metabolism*
  • Granulocyte-Macrophage Colony-Stimulating Factor / metabolism*
  • Humans
  • In Vitro Techniques
  • Interleukin-3 / metabolism*
  • Ionomycin / pharmacology
  • Neurotoxins / metabolism
  • Neutrophils / cytology
  • Neutrophils / metabolism*
  • Ribonucleases*
  • Tetradecanoylphorbol Acetate / pharmacology


  • Interleukin-3
  • Neurotoxins
  • Ionomycin
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • Eosinophil-Derived Neurotoxin
  • Ribonucleases
  • Tetradecanoylphorbol Acetate