Sulforaphane Suppressed LPS-induced Inflammation in Mouse Peritoneal Macrophages Through Nrf2 Dependent Pathway

Biochem Pharmacol. 2008 Oct 15;76(8):967-73. doi: 10.1016/j.bcp.2008.07.036. Epub 2008 Aug 7.

Abstract

Sulforaphane (SFN) is a natural isothiocyanate that is present in cruciferous vegetables such as broccoli and cabbage. Previous studies have shown that SFN is effective in preventing carcinogenesis induced by carcinogens in rodents, which is related in part to its potent anti-inflammation properties. In the present study, we compared the anti-inflammatory effect of SFN on LPS-stimulated inflammation in primary peritoneal macrophages derived from Nrf2 (+/+) and Nrf2 (-/-) mice. Pretreatment of SFN in Nrf2 (+/+) primary peritoneal macrophages potently inhibited LPS-stimulated mRNA expression, protein expression and production of TNF-alpha, IL-1beta, COX-2 and iNOS. HO-1 expression was significantly augmented in LPS-stimulated Nrf2 (+/+) primary peritoneal macrophages by SFN. Interestingly, the anti-inflammatory effect was attenuated in Nrf2 (-/-) primary peritoneal macrophages. We concluded that SFN exerts its anti-inflammatory activity mainly via activation of Nrf2 in mouse peritoneal macrophages.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Anticarcinogenic Agents / pharmacology*
  • Dinoprostone / metabolism
  • Inflammation / chemically induced*
  • Inflammation / prevention & control*
  • Interleukin-1beta / metabolism
  • Isothiocyanates
  • Lipopolysaccharides / pharmacology
  • Lipopolysaccharides / toxicity*
  • Macrophages, Peritoneal / drug effects
  • Macrophages, Peritoneal / physiology*
  • Mice
  • Mice, Knockout
  • NF-E2-Related Factor 2 / deficiency
  • NF-E2-Related Factor 2 / genetics
  • NF-E2-Related Factor 2 / physiology*
  • Nitric Oxide / metabolism
  • RNA / genetics
  • RNA / isolation & purification
  • Reverse Transcriptase Polymerase Chain Reaction
  • Thiocyanates / pharmacology*
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Anticarcinogenic Agents
  • Interleukin-1beta
  • Isothiocyanates
  • Lipopolysaccharides
  • NF-E2-Related Factor 2
  • Nfe2l2 protein, mouse
  • Thiocyanates
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide
  • RNA
  • sulforafan
  • Dinoprostone