Abstract
Epothilone and its analogs are a potent new class of anticancer compounds produced by myxobacteria. Thus, in an effort to identify new myxobacterial strains producing epothilone and its analogs, cellulose-degrading myxobacteria were isolated from Korean soils, and 13 strains carrying epothilone biosynthetic gene homologs were screened using a polymerase chain reaction. A migration assay revealed that Sorangium cellulosum KYC3013, 3016, 3017, and 3018 all produced microtubule-stabilizing compounds, and an LCMS/ MS analysis showed that S. cellulosum KYC3013 synthesized epothilone A.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Cell Line, Tumor
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Cell Movement / drug effects
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Cellulose / metabolism
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Chromatography, Liquid
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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Epothilones / biosynthesis
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Epothilones / genetics*
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Humans
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Microscopy, Interference
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Molecular Sequence Data
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Myxococcales / genetics*
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Myxococcales / isolation & purification*
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Myxococcales / metabolism
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Phylogeny
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Polymerase Chain Reaction
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RNA, Ribosomal, 16S / chemistry
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RNA, Ribosomal, 16S / genetics
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Sequence Alignment
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Soil Microbiology*
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Tandem Mass Spectrometry
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Tubulin Modulators / isolation & purification
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Tubulin Modulators / metabolism
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Tubulin Modulators / pharmacology
Substances
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DNA, Bacterial
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Epothilones
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RNA, Ribosomal, 16S
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Tubulin Modulators
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Cellulose
Associated data
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GENBANK/EU242515
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GENBANK/EU242516
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GENBANK/EU242517
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GENBANK/EU242518
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GENBANK/EU242519