[Development of a multiplex PCR for detection of the Yersinia genus with identification of pathogenic species (Y. pestis, Y. pseudotuberculosis, Y. enterocolitica)]

Mol Gen Mikrobiol Virusol. 2008;(3):18-23.
[Article in Russian]

Abstract

To identify the Yersinia genus and pathogenic species (Y. pestis, Y. pseudotuberculosis, Y. enterocolitica) in a single reaction a multiplex PCR technique was developed. It was optimized by five compounds of PCR buffer and temperature of primers annealing. The multiplex PCR provides an improved and rapid method for detection of the Yersinia genus and identification of pathogenic species.

Publication types

  • English Abstract

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / genetics*
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Porins / genetics*
  • Yersinia / classification*
  • Yersinia / genetics
  • Yersinia / isolation & purification
  • Yersinia enterocolitica / classification
  • Yersinia enterocolitica / genetics
  • Yersinia enterocolitica / isolation & purification
  • Yersinia pestis / classification
  • Yersinia pestis / genetics
  • Yersinia pestis / isolation & purification
  • Yersinia pseudotuberculosis / classification
  • Yersinia pseudotuberculosis / genetics
  • Yersinia pseudotuberculosis / isolation & purification

Substances

  • Bacterial Proteins
  • Porins