Cloning of a Human Gene Encoding the General Transcription Initiation Factor IIB

Nature. 1991 Aug 22;352(6337):689-95. doi: 10.1038/352689a0.

Abstract

Transcription factor IIB (TFIIB) has a central role in transcription of class II genes. The purification of the human TFIIB protein and isolation of a complementary DNA encoding TFIIB activity is reported here. The sequence of TFIIB, which seems to be encoded by a single gene, contains a repeated motif, in addition to a motif with similarity to the prokaryotic sigma-factors. The recombinant protein expressed in bacteria substituted for all the functions attributed to the human TFIIB protein.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • DNA / genetics
  • DNA-Binding Proteins / chemistry
  • Humans
  • Molecular Sequence Data
  • Oligonucleotides / chemistry
  • Protein Conformation
  • Solubility
  • Transcription Factor TFIIB
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / isolation & purification
  • Transcription, Genetic

Substances

  • DNA-Binding Proteins
  • Oligonucleotides
  • Transcription Factor TFIIB
  • Transcription Factors
  • DNA

Associated data

  • GENBANK/X57450
  • GENBANK/X57451
  • GENBANK/X57452
  • GENBANK/X57453
  • GENBANK/X57454
  • GENBANK/X57455
  • GENBANK/X57688
  • GENBANK/X57689
  • GENBANK/X59268
  • GENBANK/X59436