Modulation of Redox Status in Human Lung Cell Lines by Organoselenocompounds: Selenazolidines, Selenomethionine, and Methylseleninic Acid

Toxicol In Vitro. 2008 Oct;22(7):1761-7. doi: 10.1016/j.tiv.2008.08.003. Epub 2008 Aug 15.

Abstract

Cancer prevention strategies utilizing selenium-containing compounds have demonstrated reduced cancer mortality and efficacy for some cancer types but considerable differences in cellular effects exist among the selenocompounds employed. The variability of the effects on cell viability, redox modulation, and disruption of subcellular compartments by the conventional selenium-containing amino acid, selenomethionine, the oxidized selenosugar metabolite, methylseleninic acid, and selenazolidines was investigated in A549 and BEAS-2B human lung cell lines. Selenomethionine had little effect whereas methylseleninic acid increased cellular thiols and stress in the endoplasmic reticulum. The cyclohexylselenazolidine increased mild oxidative stress in the adenocarcinoma cell line, A549, but the effects were attenuated in the normal, but virally transformed cell line, BEAS-2B. These data demonstrate that all selenocompounds are not equal and that the form of the organic selenocompound is a major determinant in the expected cellular response.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cell Line, Transformed
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Endoplasmic Reticulum / drug effects
  • Endoplasmic Reticulum / metabolism
  • Humans
  • Lung Neoplasms / drug therapy
  • Lung Neoplasms / metabolism
  • Organoselenium Compounds / chemistry
  • Organoselenium Compounds / pharmacology*
  • Oxidation-Reduction / drug effects*
  • Oxidative Stress / drug effects
  • Selenomethionine / pharmacology*
  • Sulfhydryl Compounds / metabolism

Substances

  • Organoselenium Compounds
  • Sulfhydryl Compounds
  • Selenomethionine
  • methylselenic acid