Influences of reduced expression of maternal bone morphogenetic protein 2 on mouse embryonic development

Sex Dev. 2008;2(3):134-41. doi: 10.1159/000143431. Epub 2008 Sep 3.


Bone morphogenetic protein 2 (BMP2) was originally found by its osteoinductive ability, and recent genetic analyses have revealed that it plays critical roles during early embryogenesis, cardiogenesis, decidualization as well as skeletogenesis. In the course of evaluation of the conditional allele for Bmp2, we found that the presence of a neo cassette, a selection marker needed for gene targeting events in embryonic stem cells, in the 3' untranslated region of exon 3 of Bmp2, reduced the expression levels of Bmp2 both in embryonic and maternal mouse tissues. Some of the embryos that were genotyped as transheterozygous for the floxed allele with the neo cassette over the conventional null allele (fn/-) showed a lethal phenotype including defects in cephalic neural tube closure and ventral abdominal wall closure. The number of embryos exhibiting these abnormalities was increased when, due to different genotypes, expression levels of Bmp2 in maternal tissues were lower. These results suggest that the expression levels of Bmp2 in both embryonic and maternal tissues influence the normal neural tube closure and body wall closure with different thresholds.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Abdominal Wall / abnormalities
  • Abdominal Wall / embryology
  • Animals
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins / genetics*
  • Bone Morphogenetic Proteins / metabolism
  • Embryo, Mammalian
  • Embryonic Development / genetics*
  • Embryonic Development / physiology
  • Female
  • Genes, Lethal
  • Germ-Line Mutation / physiology
  • Maternal-Fetal Exchange / genetics*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Mothers*
  • Neural Tube Defects / embryology
  • Neural Tube Defects / genetics
  • Organ Specificity / genetics
  • Pregnancy
  • Survival
  • Transforming Growth Factor beta / genetics*
  • Transforming Growth Factor beta / metabolism
  • Transgenes


  • Bmp2 protein, mouse
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Transforming Growth Factor beta