Abstract
This unit describes the use of satellite tobacco mosaic virus (STMV) vectors in combination with native TMV particles for inducing transient gene silencing in tobacco plants. Target gene fragment selection and insertion, virus delivery procedures, and phenotype screening of silenced plants are described in detail. All critical parameters for tobacco plant cultivation, virus infection, and RNA silencing efficiency are discussed.
MeSH terms
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Gene Silencing*
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Gene Targeting
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Genetic Techniques*
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Genetic Vectors / genetics*
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Helper Viruses / genetics
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Helper Viruses / isolation & purification
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Nicotiana / genetics
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Nicotiana / growth & development
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Nicotiana / virology*
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Phenotype
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Plant Diseases / virology
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Plant Leaves / genetics
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Plant Leaves / virology
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Plants, Genetically Modified / genetics
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Tobacco Mosaic Virus / genetics
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Tobacco Mosaic Virus / isolation & purification
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Tobacco mosaic satellite virus / genetics*