Flow cytometric analysis of mitochondrial membrane potential using JC-1

Curr Protoc Cytom. 2001 May:Chapter 9:Unit 9.14. doi: 10.1002/0471142956.cy0914s13.


Changes in membrane potential have long been known to represent early activation events. This unit presents very recent developments in both fluorescent probes and functional applications and demonstrates the use of the JC-1 probe for measuring mitochondrial membrane potential by flow cytometry. A valuable component of this measurement system is the possibility of making quantitative measurements of changes in membrane potential.

MeSH terms

  • Animals
  • Apoptosis
  • Benzimidazoles / pharmacology*
  • Carbocyanines / pharmacology*
  • Cell Line, Tumor
  • Cell Separation / methods*
  • Flow Cytometry / methods*
  • Fluorescent Dyes / pharmacology
  • Humans
  • Membrane Potential, Mitochondrial*
  • Mitochondria / metabolism


  • Benzimidazoles
  • Carbocyanines
  • Fluorescent Dyes
  • 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolocarbocyanine