Whole blood analysis of leukocyte-platelet aggregates

Curr Protoc Cytom. 2003 May;Chapter 6:Unit 6.15. doi: 10.1002/0471142956.cy0615s24.

Abstract

In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. The platelet binding is initiated primarily through platelet surface expression of P-selectin (CD62P) following activation-dependent degranulation. The levels of P-selectin involved can be low enough to make direct measurement difficult, but detection of leukocyte-platelet aggregates is relatively simply by whole-blood flow cytometry. Light scatter and at least one leukocyte-specific antibody are used to gate the desired population, and the presence of associated platelets is detected by immunostaining for abundant platelet-specific markers.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Blood
  • Blood Platelets / cytology*
  • Blood Platelets / immunology
  • Cell Adhesion*
  • Flow Cytometry / methods*
  • Humans
  • Immunohistochemistry
  • Leukocytes / cytology*
  • Leukocytes / immunology
  • Methods

Substances

  • Antibodies, Monoclonal