Boosting tandem affinity purification of plant protein complexes

Trends Plant Sci. 2008 Oct;13(10):517-20. doi: 10.1016/j.tplants.2008.08.002. Epub 2008 Sep 2.


Protein-interaction mapping based on the tandem affinity purification (TAP) approach has been successfully established for several systems, such as yeast and mammalian cells. However, relatively few protein complex purifications have been reported for plants. Here, we highlight solutions for the pitfalls and propose a major breakthrough in the quest for a better TAP tag in plants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Carrier Proteins / chemistry
  • Endopeptidases / chemistry
  • Plant Proteins / isolation & purification*
  • Protein Interaction Mapping / methods*


  • Bacterial Proteins
  • Carrier Proteins
  • IgG Fc-binding protein, Streptococcus
  • Plant Proteins
  • streptavidin-binding peptide
  • Endopeptidases
  • TEV protease