Methods for studying mechanical control of angiogenesis by the cytoskeleton and extracellular matrix

Methods Enzymol. 2008;443:227-59. doi: 10.1016/S0076-6879(08)02012-0.


Mechanical forces that capillary endothelial cells generate in their cytoskeleton and exert on their extracellular matrix adhesions feed back to modulate cell sensitivity to soluble angiogenic factors, and thereby control vascular development. Here we describe various genetic, biochemical, and engineering methods that can be used to study, manipulate, and probe this physical mechanism of developmental control. These techniques are useful as in vitro angiogenesis models and for analyzing the molecular and biophysical basis of vascular control.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cell Cycle / physiology
  • Cell Shape / physiology
  • Cells, Cultured
  • Cytoskeleton / metabolism*
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism
  • Endothelial Cells / physiology
  • Extracellular Matrix / metabolism*
  • Humans
  • Mice
  • NIH 3T3 Cells
  • Neovascularization, Physiologic / physiology*
  • Stress, Mechanical*